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A re-usable wave bioreactor for protein production in .

Having Labfors stirred-tank bioreactors and Wave Platforms available, we invested efforts in bioreactor cultivation. Upscale to 5 L was first performed in a stirred-tank bioreactor (Labfors, Infors, 7.5 L) adapted to insect-cell cultivation with integrated pH and pO 2 measurement and regulation. Expression levels were comparable to flask .

The bioreactor: a powerful tool for large-scale culture of .

Bioreactors play a key role in the field of biologics, where they are used for the production of recombinant therapeutic proteins by large-scale cultivation of animal cells. There are several types of bioreactors, including stirred-tank, airlift, hollow-fiber, and Rotary Cell Culture System (RCCS) designs.

Culture of human T cells in stirred bioreactors for .

Mar 31, 2000 · R. Eibl, D. Eibl, Application of Disposable Bag Bioreactors in Tissue Engineering and for the Production of Therapeutic Agents, Bioreactor Systems for Tissue Engineering, 10.1007/978-3-540-69357-4_8, (183-207), (2009).

Used Bioreactors | Buy & Sell | EquipNet

EquipNet is the world's leading provider of used bioreactors and various other preowned equipment. Our exclusive contracts with our clients yield a wide range of used bioreactors from a number of respected OEMs, including Wave Biotech, Thermo Scientific, B. Braun Biotech, New Brunswick Scientific, Precision Stainless, Xcellerex, Lee Industries, and many more.

WAVE BIOREACTOR CHARACTERIZATION: RESIDENCE TIME .

innovative mechanism of rocking of the Wave bioreactor, associated with its recentness, results in the need for a better characterization of the reactor operation. This will allow the know-how concerning Wave to become comparable to that of more common bioreactors, such as the stirred tank reactor (STR). One

US20050239198A1 - Stirred-tank reactor system - Google Patents

US20050239198A1 US11/064,252 US6425205A US2005239198A1 US 20050239198 A1 US20050239198 A1 US 20050239198A1 US 6425205 A US6425205 A US 6425205A US 2005239198 A1 US2005239198 A1 US 2005239198A1 Authority US United States Prior art keywords bag stirred tank reactor reactor system system Prior art date 2004-04-27 Legal status (The legal status is an .

309. Plasmid DNA Production in a Wave Bioreactor under .

To mimic the aeration and agitation of the stirred tank the wave angle and rate were set to their practical maxima. The mean specific yield (mg of plasmid / g wet weight) obtained from the wave bioreactor was found to range from equivalent to 3 fold higher than that obtained from the same bacterial master cell bank in the stirred tank fermentor .

Comparing wave and stirred-tank bioreactor performance for .

Based on these results further experiments for virus propagation in stirred-tank-(5 L-scale) and wave bioreactor (1 L-scale) were carried out. Both culture vessels are compared with respect to cell growth and virus yields. We are able to show that both types of bioreactors are suitable for use in MEV vaccine production process.

Bioreactors Types: Top 3 Types of Bioreactors

ADVERTISEMENTS: This article throws light upon the three types of bioreactors. The three types of bioreactors are: (1) Mechanically Agitated Bioreactors (2) Pneumatically Agitated Bioreactors and (3) Wave Bioreactors. Type # 1. Mechanically Agitated Bioreactors: The mechanically agitated bioreactors are most commonly used for large-scale culture of plant, animal and microbial cells. In .

Efficient High-Titer Monoclonal Antibody Production In A .

This application note shows the feasibility of monoclonal antibody (MAb) production in fed-batch processes using the single-use Xcellerex™ XDR-200 stirred-tank and ReadyToProcess WAVE™ 25 rocking bioreactor systems. Cell expansion was performed in a 15 L rocking bioreactor culture to seed a 200 L stirred-tank bioreactor culture.

Evaluation of a Single-Use Bioreactor for the Fed-Batch .

Compared to traditional wave bioreactors, stirred-tank bioreactors offer the benefits of sparging, stirring of the suspension, and a higher utilization rate of the bag size as cultivation space. Such bioreactors are considered for use in the industry in cell amplification processes, to reach higher cell densities, or as production bioreactors.

Novel biomanufacturing platform for large-scale and high .

Apr 23, 2019 · First, the perfusion culture in WAVE takes 18 days to expand by > 100 folds from stimulation to harvest, [52, 53], but our stirred-tank bioreactor can achieve 100-fold expansion within 8 days. Second, the agitation and gas sparging in the stirred-tank bioreactor offered optimal oxygen transfer, nutrients transfer, and a more homogenous .

Evaluation of a Single-Use Bioreactor for the Fed-Batch .

Compared to traditional wave bioreactors, stirred-tank bioreactors offer the benefits of sparging, stirring of the suspension, and a higher utilization rate of the bag size as cultivation space. Such bioreactors are considered for use in the industry in cell amplification processes, to reach higher cell densities, or as production bioreactors.

Biostat® RM - Wave-Mixed Bioreactor | Sartorius

BIOSTAT® RM 20 | 50 basic is a perfectly sized, single-use, wave-mixed benchtop bioreactor for stand-alone use. It features an exchangeable bag holder to fit bags with a total volume of 1 – 50 L. The BIOSTAT® RM Basic rocking platform with an integrated local controller, Air | CO2 mixing module and optional load cells is the optimal choice .

GE Wave Bioreactor System

The GE WAVE Biotech 2/10EH Bioreactor is a flexible system that can be used at the work bench or inside an incubator. This bioreactor comes with a 10L cell bag holder set on a rocking base with integrated temperature control. This bioreactor has a rocking speed range of 3 - 40 rocks per minute at an adjustable angle between 2° - 9°. The temperature range of this cell culture bioreactor is 15 .

Bioreactor and Fermentor - The Lab World Group

The GE Wave Bioreactors and Sartorius Biostat Wave Bioreactors design leverages single-use, closed system cell growth bags, and are modular, easy to clean and have digital control for a smaller scale, flexible option. While the stirred tank Chemglass systems allow users to scale up their culture volume production levels.

Single Use Bioreactors Market by Product, Type, End User .

7 Single use Bioreactor Market, By Type (Page No. - 63) 7.1 Introduction 7.2 Stirred-Tank Single use Bioreactor 7.3 Wave-Induced Single use Bioreactor 7.4 Bubble-Column Single use Bioreactor 7.5 Other Single use Bioreactor . 8 Single use Bioreactor Market, By Type of Cell (Page No. - 73) 8.1 Introduction 8.2 Mammalian Cells 8.3 Bacterial Cells

(PDF) Application of different types of bioreactors in .

This chapter presents bioreactors models applied to bioprocesses including submerged (stirred tank reactor, bubble column, air-lift, membrane, packed bed and fluidized bed bioreactors) and solid .

Mammalian | Cytovance® Biologics — Cytovance Biologics

Cell propagation in stirred-tanked bioreactors, flasks, Wave® Bioreactor, HYPERStack® Cell Culture Vessel, roller bottles, and spinner flasks. Media screening/optimization. Feed media screening and feed strategies development. Adaptation of cell lines to chemically defined, protein free and animal component free cGMP compliant media

Eppendorf New Brunswick CelliGen BLU Bioreactor

The Eppendorf New Brunswick CelliGen BLU BioReactor provides single-use technology in a stirred tank design, for the best in performance, scalability and convenience. This bioreactor is built for high density animal cell culture research and production, with the ability to handle up to 32 processes parameters and can trend up to 8 loops. The bright, easy to read touch screen display gives you .

Bioreactor and Fermentor Market Trends

Aug 01, 2009 · Compared to a mass transfer coefficient (kLa) of . 15/h for a wave-type reactor, and about 30/h for a typical stirred tank reactor, the CELL-tainer can achieve a kLa 200/h and with oxygen up to .

Comparing wave and stirred-tank bioreactor performance for .

Based on these results further experiments for virus propagation in stirred-tank-(5 L-scale) and wave bioreactor (1 L-scale) were carried out. Both culture vessels are compared with respect to cell growth and virus yields. We are able to show that both types of bioreactors are suitable for use in MEV vaccine production process.

Keys to successful single-use bioreactor scale up – from .

Successful monoclonal antibody (mAb) manufacturing relies on good process development and reliable bioreactor scale up. Initial process development work is typically conducted at small scale, then moved to bench scale and lastly to pilot scale before moving into manufacturing. One of the biggest challenges is scaling up an optimized process from process development to manufacturing scale.

Scale-Up and Comparison Studies Evaluating Disposable .

Originally, cell culture was performed in WAVE bioreactors (GE Healthcare). However, because of limitations on control and scalability, a more robust system became necessary. The stirred-tank single-use bioreactor (SUB) by HyClone (Thermo Fisher) provided a stirred-tank design with the ability to control, monitor, and log data throughout cell .

Application note 29-1193-76 AA Cell culture Efficient .

ReadyToProcess WAVE™ 25 rocking bioreactor systems. Cell expansion was performed in a 15 L rocking bioreactor culture to seed a 200 L stirred-tank bioreactor culture. To compare the performance of ReadyToProcess WAVE 25 with that of XDR-200, 7 L of the 200 L culture was transferred to a parallel ReadyToProcess WAVE 25 system

Fermenters and Bioreactors | New and Used Lab Fermenters .

Applikon Bioreactor B. Braun Fermentor Bioprocess System CelliGen Bioreactor Dasgip Parallel Bioreactor EPPENDORF BioFlo 320 EPPENDORF DASGIP DASbox Eppendorf Bioreactor GE Healthcare ReadyToProcess WAVE 25 New Brunswick Bioflo 3000 Reactors and Fermentors New Brunswick Bioflo Reactors and Fermentors New Brunswick Scientific BioFlo 310

MDCK and Vero cells for influenza virus vaccine production .

scale cultivations were evaluated in a stirred tank and a wave bioreactor using two different media. Materials and methods Cell lines Adherent MDCK cells (ECACC 84121903) and adherent Vero cells (WHO seed; ECACC 88020401) were either cultivated under serum-containing conditions (GMEM # 22100-093, Gibco) as described by Genzel et al.2004)

Biostat STR® Generation 3 Single-Use Bioreactor | Sartorius

Biostat STR® bioreactors and Flexsafe STR® bags range from 50L to 2000L and can help you achieve outstanding speed, quality, and flexibility in your process development and commercial manufacturing operations. Accelerate your success with a stable, predictable process that delivers a safe, reliable biologic medicine for patients around the .

Application note 29-1193-76 AA Cell culture Efficient .

ReadyToProcess WAVE™ 25 rocking bioreactor systems. Cell expansion was performed in a 15 L rocking bioreactor culture to seed a 200 L stirred-tank bioreactor culture. To compare the performance of ReadyToProcess WAVE 25 with that of XDR-200, 7 L of the 200 L culture was transferred to a parallel ReadyToProcess WAVE 25 system

Upstream Continuous Process – Perfusion Culture - Cell .

Jun 23, 2018 · Mikola et al. examined a WAVE bioreactor with a 10-L bag filled to 50% volume with phosphate-buffered saline (PBS) and UCON antifoam at 30°C (4). When the system rocked at a 10° angle, k L a was linearly dependent on rocking rates up to 25 rpm but increased by a factor of four when the rocking rate was 40 rpm.